Enhanced protein expression in the baculovirus/insect cell system using engineered SUMO fusions
نویسندگان
چکیده
منابع مشابه
Engineered Saccharomyces cerevisiae strain for improved xylose utilization with a three-plasmid SUMO yeast expression system.
A three-plasmid yeast expression system utilizing the portable small ubiquitin-like modifier (SUMO) vector set combined with the efficient endogenous yeast protease Ulp1 was developed for production of large amounts of soluble functional protein in Saccharomyces cerevisiae. Each vector has a different selectable marker (URA, TRP, or LEU), and the system provides high expression levels of three ...
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protein secretory systems are found in all living organisms including gram negative bacteria, and eukaryotic cell’s organelles that are derived from these bacteria. unlike all other living organisms, gram negative bacteria have different protein secretory systems. at least there are four different independent secretory systems, which are responsible to transport the secreted proteins to the mil...
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ژورنال
عنوان ژورنال: Protein Expression and Purification
سال: 2008
ISSN: 1046-5928
DOI: 10.1016/j.pep.2008.07.010